Qikun Jia

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Questions related from Qikun Jia

Primers designed by cDNA could not result any band as genomic DNA as template

To get a gene's full sequence, I design some primers according to the RNAseq result and cDNA-RACE. it should be note that RNAseq result is consistent with cDNA-RACE result. i try to make the...

06 June 2017 438 4 View

How to choose a specific gene that could used to determine one specie exsit or not by PCR?

I want to determine one contaminant exsit or not in my cultivation by PCR. However, I am not sure is the primers targeted in 16S sequence designed by NCBI specific enough to support my...

10 October 2016 7,385 3 View

Why the C. reinhardtii (CW15) cell crushed seriously when we replace the TAP meidum with nitrogen free TAP?

I am trying to replace the TAP medium with N-free TAP medium after the CW15 has been cultured in TAP medium for 3 days with centrifuge at 1500 g for 5 min followed by pipetting sever time and...

06 June 2016 8,253 2 View