Qikun Jia

5 Questions 8 Answers 0 Followers

Questions related from Qikun Jia

How can it been contaminated since the Bst enzyme had been inactived in LAMP reaction?

all the manual or tips about LAMP think that false positive will hapen if you open the PCR tube for gel electrophoresis detection. How can it been for the enzyme has been inactived by 85 degree...

27 July 2017 7,284 1 View

Primers designed by cDNA could not result any band as genomic DNA as template

To get a gene's full sequence, I design some primers according to the RNAseq result and cDNA-RACE. it should be note that RNAseq result is consistent with cDNA-RACE result. i try to make the...

14 June 2017 308 3 View

How to choose a specific gene that could used to determine one specie exsit or not by PCR?

I want to determine one contaminant exsit or not in my cultivation by PCR. However, I am not sure is the primers targeted in 16S sequence designed by NCBI specific enough to support my...

12 October 2016 9,894 3 View

Why the C. reinhardtii (CW15) cell crushed seriously when we replace the TAP meidum with nitrogen free TAP?

I am trying to replace the TAP medium with N-free TAP medium after the CW15 has been cultured in TAP medium for 3 days with centrifuge at 1500 g for 5 min followed by pipetting sever time and...

17 June 2016 1,530 2 View

Is there some inverted repeat in the nuclear sequence in chlamydomonas reinhardtii?

I found that the overexpression of gene in C. reinhardtii always use the non homologous random insertion. I just wonder is that OK to inserted a gene into the chromosome of C. reinhardtii with the...

25 September 2015 2,212 2 View