Qian Yee Wong

2 Questions 1 Answers 0 Followers

Questions related from Qian Yee Wong

Why protein ladder starts separating in sds-page stacking gel?

I made two 10% sds-page gel (10% resolving and 4% stacking) and run them at 20mA. I am testing whether my newly edited recipe for gel casting is correct or not. Apparently, the gels took 3hr for...

14 December 2023 7,450 1 View

A line appeared on the SDS-PAGE gel, what is the cause?

I am optimising a new protocol for SDS-PAGE using 40% acrylamide 29:1 solution from Bio-Rad across different percentages. I casted two 6% equivalent gels according to the protocol I have...

09 November 2023 6,887 0 View