Qari Muhammad Imran

5 Questions 12 Answers 0 Followers

Questions related from Qari Muhammad Imran

What will be the effect (on gene expression/protein) of a genomic DNA contamination while generating 35S OX construct?

i need your expert opinion. if we are cloning a gene which is amplified by a cDNA that is having some traces of gDNA. Following TA cloning and sub cloning into a destination vector or protein...

01 January 2018 2,192 4 View

How can i clone a 3.1 kb gene in any destination vector for agrobacterim transformation?

i am cloning a 3.1 kb long gene in pEarlyGate 103 and pEarlyGate using Gateway cloning system but i am unable to clone it. before i cloned successfully less than 3 kb genes. is the size of this...

02 February 2016 2,719 3 View

How can I get increase concentration of Plasmid containing my target gene?

i am doing some transformation work in Arabidopsis thaliana and ordered cDNA clone from ABRC stock center. i want to confirm the sequence for which i am extracting plasmid with "Cosmo GENETICH"...

04 April 2015 6,272 6 View

How much plasmid should we load on a gel to check its quality?

I want to confirm my transformed construct in pEarleyGate 201 and 103, but first I want to check the plasmid on gel. what should be optimum amount to load

03 March 2015 5,763 8 View

I am having very low concentration of RNA using trizol method from Arabidopsis leaves, any suggestion and optimized protocol?

i am using trizol reagent method for RNA extraction from Arabidopsis leaves, but the concentration is very low in some samples 1ng/ul. if any one can give some suggestions or can give an optamized...

08 August 2014 2,705 7 View