Pushpa Kumari

3 Questions 8 Answers 0 Followers

Questions related from Pushpa Kumari

Non-specific labelling with Click chemistry reaction?

I am working on identification of a small peptide in brain tissue. We have modified the peptide with alkyne modifications for azide-based clicking. For Click reaction we have used 50 uM CuSO4,...

09 September 2019 8,591 2 View

Primary Hippocampal culture contamination.

Currently I am doing Low density hippocampal cultures. The neurons are growing nicely, and with very little cell debris.The problem that I am facing is that they are getting easily contaminated. I...

04 April 2013 5,044 3 View

Background in western blot?

Hi all, I am facing a problem in getting cleaner blot using an antibody against Polyubiquitin conjugates (FK1, Enzo life science) from an Immunoprecipitated sample. I have used both 5% Milk or BSA...

01 January 1970 5,187 9 View