How can we liquify sputum sample to aim efficient M.tb DNA extraction from sputum sample? Can we extract Mtb DNA from sputum treated with NaOH-NALC?

I am currently facing difficulty in the DNA extraction because of the viscosity of the sputum samples. I am using the Chelex based extraction method which does yield in DNA but it is inefficient...

12 December 2019 3,248 1 View