Punith B D

3 Questions 6 Answers 0 Followers

Questions related from Punith B D

Which is the best method/algorithm for phylogenetic comparison of protein coding sequences (ORF) and amino acid sequences?

I need compare a particular protein coding (nucleotide) sequence and amino acid sequence with vertebrates and non-vertebrates and understand the mechanisms of evolution and relatedness using...

04 December 2016 3,449 3 View

What is a good culture medium for establishing primary hepatocytes? Why should phenol red not be used in hepatocyte culture?

If anyone has done primary liver cell culture please suggest which is the good culture medium for primary hepatocytes and also tell me prospects for using DMEM or DMEM-Ham's F12. Also please...

09 June 2014 1,764 12 View

Does anyone have a protocol for in-vitro differentiation of bone marrow mesenchymal stem cells to hepatocytes?

So far I get the protocols and the research articles which describe the differentiation of mesenchymal stem cells into hepatocyte like cells, but not hepatocytes exactly. Also please suggest me...

09 June 2014 2,335 2 View