Pujitha Lakshmi R

2 Questions 3 Answers 0 Followers

Questions related from Pujitha Lakshmi R

Hi, What is the basic difference between an ultramer and a gblock?

I am planning to overexpress an sRNA in K.pneumoniae (inherently resistant to Amp, hence cannot use pUC19 because of it Amp resistance and not useful to scree). Which is better to clone in an...

15 November 2020 1,716 3 View

Can I ligate a blunt end of insert with sticky end of a vector ?

I am trying to ligate an insert that has Sca1 (blunt) and Xho1 (sticky) as 2 flanks with a vector that is cut with Sal1 (sticky) and sma1 (blunt). Does these ligate considering the sticky-blunt...

10 September 2020 826 4 View