Puja Gupta

3 Questions 5 Answers 0 Followers

Questions related from Puja Gupta

How long for should I stimulate mouse spleen cells with Concanavalin A?

Does anyone know how many mouse spleen cells should be stimulated with how much Concanavalin A? How for long to see a considerable number of spots?

03 March 2015 458 10 View

What positive control should I use for murine IFN gamma in ELISPOT?

Hi! I am doing ELISPOT for the first time has no previous experience. I want to test mouse spleen cells injected with peptides. Right now I am trying to make my positive control work. I am using...

10 October 2014 4,574 3 View

Why I am seeing these black streaks in ELIspot under dissecting microscope?

Hi! All, I am doing ELIspot for the first time and am having problems getting spots even with concanavalin stimulated spleen cells. I am seeing spots with the naked eye but when I look under a...

10 October 2014 1,883 12 View