Prranshu Yadav

4 Questions 4 Answers 0 Followers

Questions related from Prranshu Yadav

Can someone please explain why I am getting this irregular and wavey band pattern in SDS-PAGE?

The sample I loaded is yeast cell lysate lysed by sonication.

11 November 2016 5,487 3 View

How can I get rid of a LYS2 marked plasmid in yeast?

I have a yeast strain containing a LYS2 marked plasmid, which makes it prototrophic for lysine. Without the plasmid, it would be auxotrophic for lysine. I would like to know if there is any method...

07 July 2014 7,088 2 View

For how long can plasmids be stored in 100% Isopropanol at -20 degrees Celsius?

I have heard that plasmids can be stored in isopropanol for years because they remain very stable in it. Any suggestions?

02 February 2014 1,712 9 View

Is it possible to isolate plasmids from a contaminated E coli culture?

My E coli culture has started showing some clumps after repeated innoculations and I suspect it is contaminated. I do not have another culture containing my plasmid.

12 December 2012 734 11 View