Does anybody know the protocol for making electrocompetent cells of geobacillus and its electroporation?

I am having problem in transforming pNW33N plasmid, a low-copy plamsid in geobacillus strain. This plasmid propogates at 50-55 degree C but after plating and incubating at 50 degree C for 12-15...

10 October 2014 8,278 3 View