Priyanka kriti Narayan

3 Questions 4 Answers 0 Followers

Questions related from Priyanka kriti Narayan

How to analyse 3D DNA FISH data?

I have done 3D DNA FISH of two gene probed with alexa fluor 488 and alexa fluor 594 respectively and counter staining with DAPI with good signal to background ratio and I want to calculate the...

13 July 2019 7,895 2 View

Protocol for Gene loci fluorescence in situ hybridization in suspension cells.

I am working on gene loci 3-D DNA FISH in suspension cells(primary lymphoid cells), but it's not working and I am not able to troubleshoot.I have tried to execute the experiment many times but I...

10 May 2017 419 3 View

Hello everyone, How to separate protein with the molecular mass difference of 3 kDa utilizing SDS-PAGE?

The idea here is to biotinylate the transcription factor X in vivo with the help of acceptor peptide and BirA in X-/- cell. The molecular weight of protein after tagging and biotinylation is 67...

27 March 2017 1,490 4 View