Is RBS and TATA BOX necessary for protein expression between heavy and light chain during a mab cloning in pcDNA 3.1 VECTOR?

I am expressing a Mab in mammalian cells. Is it necessary to incorporate a Kozak sequence before the ATG initiation codon to assure that the expression happens? And also do I need to add an extra...

08 August 2018 4,319 0 View