Priyanka Mishra

9 Questions 5 Answers 0 Followers

Questions related from Priyanka Mishra

Which bacteria can grow on 10x PBS buffer?

I have prepared PBS buffer and components for M9 medium and I store it in freeze, I do not use it if it is more than 2 weeks old. From time to time I check the sterility of these solutions and...

05 November 2020 9,658 6 View

Why Pseudomonas fluorescens ATCC13525 does not grow on acetate?

I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...

21 February 2020 2,601 6 View

Is raman peak at 515cm-1 normal for silicon wafer with 532 laser and 600 grating?

We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...

03 December 2019 5,683 3 View

What are some of the best substrate that we can use for Raman measurement of bacteria except CaF2?

I am looking for the cheaper substrate for Raman measurement of bacteria, without compromising much on the quality of spectra, also does anybody have experience with metal coated slide for...

04 June 2019 6,312 3 View

Which is the best way to assess the viability of biofilm?

I have cover slip sized glass slide with some Au/Ag nano-particles spot having diameter of around 50 micro meter. I want to determine the viability of the Biofilm around that spot. How can I...

15 November 2017 7,485 5 View

What should be the ideal size of Au or Ag nanoparticles for getting optimum SERS effect ?

I want to perform SERS for bacteria, i want to know what should be the ideal size of Au or Ag nano-particles which can create optimum plasmonic effect considering also the stability of the particles.

26 October 2017 3,524 1 View

Can we use glassy carbon as the substrate for Raman Microscopy?

Want to study the behavior of bacteria on glassy carbon, so i wanted to know if i can directly use glassy carbon slide as substrate for Raman microbial analysis??

18 January 2017 6,354 1 View

Which is the best suited nanoparticle for SERS of bacteria?

I want to use either of gold or silver nanoparticle for SERS of bacteria. Which of them can yield optimum enhancement for bacteria? Is there really any big scientific difference between gold and...

21 June 2016 7,137 9 View

How to measure the Zeta potential of Bacteria?

Hello friends, I tried to measure zeta potential of bacteria in 10 mM PBS by malvern zetasizer, but during measurement both the electrodes turned black and my bacterial culture precipitated and...

02 February 2016 703 6 View