Priyanka G Bhosale

4 Questions 14 Answers 0 Followers

Questions related from Priyanka G Bhosale

How can I remove a non specific band at a particular molecular weight in western blotting?

The protein of my interest is of 69 k Da, but i observe a nonspecific band at 55 Kda along with the specific band, even after increasing blocking i see this band, it also stains ladder at same...

19 September 2014 4,090 4 View

Can anyone suggest best transfection method (non viral) for SCC29B (buccal mucosa cell line)?

I want to down-regulate gene of interest in SCC cells but this cells are hard to transfect. I have tried using Xtreme gene as well but got 10-15% transfection.

04 August 2014 9,210 3 View

Is it possible to determine the reason why one of my genes of interest is down-regulated, eg promoter methylation or something else?

In my study on human tumors, samples showed down regulation of one of my genes of interest. I am interested to know whether its because of promoter methylation or transcriptional inhibition. How...

18 October 2012 3,057 8 View

What is the best fixative for cryosectioned tissues, that can maintain DNA and protein stability?

Till date we used methanol acetic acid based fixative on cryosetioned (5 micron sections) human tissues and stored them at -20, so that it can be used for Florescent In situ Hybridization, Now...

29 September 2012 3,052 8 View