Priya Ramanathan

3 Questions 6 Answers 0 Followers

Questions related from Priya Ramanathan

Can someone please suggest cell lines that can generate exosomes efficiently when transfected with an miRNA- vector system?

I would like to use miRNA or anit-miRNA for a particular target by packaging them into exosomes and delivering them to DCs. But first I need to generate the exosomes for which I want to use cell...

09 September 2019 2,639 2 View

Could anybody share their experience with flurophore labeling kits that are commercially available?

We have with us unconjugated primary antibodies that are compatible with flow cytometry and western blot. Since we are using a panel of antibodies We would like to label these for flow instead of...

05 May 2015 3,701 4 View

Is it ok to freeze cells for later use after staining and fixing for FACS? If so can somebody share the protocol?

We do a lot of Immunophenotyping and would like to use some samples which are positive for rare markers as controls for future experiments. Is it ok to freeze those samples after staining and...

11 November 2014 9,013 8 View