Priscilla Glenn

3 Questions 3 Answers 0 Followers

Questions related from Priscilla Glenn

Is it possible to use more than one index primer per reaction?

I am using the NEBNext Ultra II Kit for library prep. This was my first time doing a library prep and we received a trial kit. I had four samples and I used Adapter 2 from the NEBNext Multiplex...

24 February 2016 3,660 3 View

After running Tophat is there a way to remove rRNA contaminants?

I have run Tophat on several Zea mays fq files and realize that there is a small percentage of rRNA contaminants within them. I know I could have removed them earlier by using ARTseq contamination...

23 June 2015 5,920 4 View

What are the differences between RPKM and FPKM in RNA-seq?

Looking at gene expression levels and will eventually analyse the X:A ratio in both males and females.

10 April 2015 9,243 6 View