Priscila Rodríguez Rodríguez

3 Questions 5 Answers 0 Followers

Questions related from Priscila Rodríguez Rodríguez

Which Universal primer is the best to tag with fluorophores for microsatellite characterization and genotyping?

I intend to implement the method in Schuelke et al. (2000) to characterize microsatellite primers in different plant species at a lower cost. Schuelke used the M13(-21): TGTAAAACGACGGCCAGT, but I...

03 March 2018 6,910 3 View

Do you know any software that can analyze absence/presence dataset from allopolyploid microsatellite data?

Hi! I have microsatellite genotypes from an allopolyploid species. Do you know any software that can treat absence/presence data for genetic diversity analysis?

10 October 2015 7,936 5 View

How can I distinguish between polyploid individuals from errors in PCR?

Genotyping all individuals from Viola cheiranthifolia, I found that one population has three alleles for 2 or 3 of the microsatellite markers (16 in total). Repeating the DNA extraction and PCR of...

12 December 2014 5,292 4 View