How do I extract viable protien (LL-37) for ELISA from attached THP-1 cells?

I have THP-1 cells differentiated using 20ng/ml PMA and incubated in 12-well plates at 2x10^5. I would like to lyse the cells and extract LL-37 or hCAP18 from my cells to use for an human LL-37...

19 March 2024 2,197 4 View