I have to add IL-2 signal peptide in front of gene of interest in pcdna 3.1 vector to export it out of the cells after transfection. How to do that?

My Questions are: 1. Should the gene of interest be directly after the IL-2 sequence and if that is the case, should it have its own start codon (ATG)? 2, if it has to have its own ATG, would that...

03 May 2016 5,703 3 View