Premala Devaraju

3 Questions 2 Answers 0 Followers

Questions related from Premala Devaraju

Can help me to troubleshoot problem with Glucokinase and Glucose 6 Phosphate gene in PCR and qPCR gene expression?

Facing problem with Glucokinase and Glucose 6 Phosphate gene in PCR and qPCR gene expression. I have design the primers as per table attached. I have try to run gradient PCR temp between 54-64°C...

26 January 2024 9,392 2 View

How can I accurately measure glucose concentration in culture media?

I have treated HK-2 Cells with glucose concentration (30mM,64mM), may I know how should we determine that the cell actually absorb the glucose that added into the media?

14 December 2023 1,027 1 View

How to determine stable reference genes using GeNorm?

May I know is anyone familiar using geNorm to selection most reliable housekeeping gene for analysis? As noted currently the software is in latest version (qbase+) and doesn't require to login or...

05 December 2023 1,593 0 View