Preetha Rajendiran

4 Questions 1 Answers 0 Followers

Questions related from Preetha Rajendiran

How do I extract protein from a fixed mice organ?

Due to change of plans, I need to extract protein from my samples which are kidney, heart and liver of mice. I’ve fixed them in formalin and then did a series of various concentration of sucrose...

05 August 2025 838 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

25 February 2021 9,123 3 View

How to go about designing primers?

I am working on gene sequencing for an organism that has little to none sequencing done. I have acquired the homologous sequence for that particular gene across three species. I would like to know...

25 August 2020 6,581 3 View

I have RNA sequencing data which i would like to analyse for expressions, which software should i use to study the gene expression?

this is my first time doing bioinformatics, so a user friendly software would really be good. Thank you.

09 June 2020 6,982 15 View