Prayatna Sharma

2 Questions 3 Answers 0 Followers

Questions related from Prayatna Sharma

Why is there no protein expression in BL21 cells even though the clones are positive?

Hello. I need some help regarding protein expression. My cloned gene is a thermostable amylase isolated from thermophilic bacterium and is cloned and expressed in Bl21 as well as pLysS strain....

26 January 2025 9,464 6 View

Why does supernatant of bacterial isolates show no amylase activity?

My bacterial isolates show no activity (in quantitative amylase assay) when I take supernatant (24-48 hours culture centrifuged at 10000 rpm). However, the isolates are amylase positive on starch...

23 November 2021 7,658 3 View