Prawit Oang

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Questions related from Prawit Oang

Why i get tag protein and tag-recombinant protein when i perform expression ?

I have recombinant protein 30 kDa and Nus-tagged 68 kDa. After induction i found 98 kDa and 68 kDa in induced sample. why transformant E. coli BL21 Star produced incomplete protein? recombinant...

03 March 2019 8,382 4 View

What would cause a protein to degrade after added lysis buffer?

I produced recombinant βA domain of β-Integrin protein from shrimp using recombinant plasmid pGEX4T1- βA. The plasmid was transformed into E. col BL21 Star. The cells were induced by 0.5 mM IPTG....

11 November 2018 3,976 8 View