Pravin Taware

3 Questions 9 Answers 0 Followers

Questions related from Pravin Taware

How to refold the MBP fused Membrane protein?

my contract has his-MBP-Membrane protein when i tray to express it goes in to inclusion body. for purification i lysed cell pellet with 6M GuHCl and 0.15%tritonx100 then over column i exchange...

10 February 2019 5,483 5 View

How to Run guanidine hydrochloride sample in SDS gel ?

I eluted my protein(membrane protein) with 7M guanidine hydrochloride. To run a SDS gel i tried TCA precipitation and and Ethanol precipitation; in both the method protein is not precipitated

07 August 2018 5,836 15 View

I extracted the membrane protein from membrane with 0.15% of TritonX100 and eluded with same amount but same time i get dimer and same time tetramer?

after purification i added lipid into it i got tetramer the i diluted the protein then also i not get any dimer or monomer but same experiment i did before i get dimer with same concentration

28 April 2018 248 6 View