Praveen Roylawar

3 Questions 18 Answers 0 Followers

Questions related from Praveen Roylawar

How to: superoxide radical detection by spectrofluorometer?

I did Nbt staining in tomato leaves to detect superoxide radicals (Blue formazol formation). Now I want to detect the same radicals using a spectrofluorometer. Can anyone please explain to me how...

09 September 2014 1,582 0 View

Can anyone help with extraction, detection, and quantification of phenols in tomatoes?

I want to carry out quantification of phenols in tomato with respect to foliar pathogen. Please suggest the best extraction protocols and important suggestions while doing it because I never did...

06 June 2014 8,681 3 View

How can I calculate the MDA concentration in leaves (100 mg) after taking absorbance at 532 nm and 600 nm?

100 mg tomato leaf tissue from a sample was ground in liquid nitrogen homogenized in 1 ml of 5% TCA and centrifuged at 8000 rpm for 30 min at 4 C. One ml of supernatant was incubated with 4 ml of...

05 May 2014 7,507 11 View