Praveen Singh

2 Questions 1 Answers 0 Followers

Questions related from Praveen Singh

What precautions should be taken to clone second gene to a duet vector?

I am trying to clone/insert a second gene to the pRSFDuet1 vector but the second gene is not showing up in the gel after cloning. What precautions should be taken to successfully clone the second...

05 September 2021 8,291 1 View

What is ideal temperature and time for ligation of DNA with sticky end?

I am doing gene cloning for the last two weeks. I have doubts that my ligation reaction is not working properly. Kindly suggest how to validate it.

29 July 2021 5,118 4 View