Why denaturing Urea-PAGE polymerization form air sacs and it also have very high background signal during Cy5 scan?

Hi everyone, I am facing an issue with running the denaturing Urea-PAGE to visualize the digested fragment of the Oligos (the first labeled oligos are Cy2, and the second one is Cy5). First...

19 January 2023 6,406 0 View