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Questions related from Pratyusha Kasibhotla
We have performed gradient PCR for our primers (Tm:59) with temperatures ranging from 55 to 600C. In Thermocycler, we have observed a graph indicating different temperatures ranging...
03 March 2015 6,837 11 View
We are optimising primer for PCR,whose length is 20 bp,Tm 59.4,GC content 55.We have got bands,but we couldn't visualise it properly in Agarose superior grade type II(1.2% gel).I have attached my...
02 February 2015 2,755 12 View
