Pratyusha Thakur

2 Questions 3 Answers 0 Followers

Questions related from Pratyusha Thakur

Why my in vitro transcription doesn't work?

Hi everyone! I am currently doing In vitro transcription of two different RNAs, one is ~90bp size and another one ~200bp. I am using 2μg of DNA template, the reaction is kept for 3 hours at 37...

10 June 2024 4,664 5 View

How to knock out a sRNA gene from E. coli genome?

Hello everyone! I'm trying to knock out a non coding sRNA gene from E. coli genome by homologus recombination based method. I'm using Kan cassette and pSIM6 recombination system. I grow the pSIM6...

05 April 2023 4,944 2 View