Can anyone help in troubleshooting my cloning results which I am getting after sequencing ?

Hi, plz help me, I have cloned a gene of 3.5kbp, using already cloned plasmid as template, I just added 2 restriction sites and stop codon in the primers. ,in a vector of size 8.9 kbp, I have...

28 September 2015 5,901 7 View