Pratibha Verma

2 Questions 9 Answers 0 Followers

Questions related from Pratibha Verma

Can anyone help in troubleshooting my cloning results which I am getting after sequencing ?

Hi, plz help me, I have cloned a gene of 3.5kbp, using already cloned plasmid as template, I just added 2 restriction sites and stop codon in the primers. ,in a vector of size 8.9 kbp, I have...

28 September 2015 5,947 7 View

Is it advisable to do stable transfection in spermatogonial stem cells, vector piRES-AcGFP, with a CMV promoter?

My gene of interest is cloned in pIRES-ACGFP vector with CMV promoter . I am curious to know whether it will get integrated into genome and if yes than what is the mechanism? Kindly give me...

03 April 2014 4,513 2 View