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I am working on a structural protein. It is a membrane protein. I have purified the protein by affinity chromatography (my protein has histidine tag). But the yield is very low. To perform size...
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I have cleaned the S-300HR pre-packed column using the rigorous cleaning steps given in the instructions. (1/4 column volume 0.5M NaOH, followed by 4CV of water, 1/2 CV of 30% isopropanol, again...
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We're using S-300 HR (120ml column volume) pre-packed Gel filtration column connected to FPLC machine. The column is giving back pressure of above 20psi at 0.25ml/min flow rate. We've run the...
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