Can we substitute commercially available Sequencing buffer with self made buffer (400 mM Tris-HCL, 10mM MgCl2) ??

If anyone can suggest if I can use a lab made sequencing buffer of (400 mM Tris-HCL, 10mM MgCl2 and pH 9) in place commercial Sequecing buffer while performing Cyclic sequencing. How does the...

12 December 2019 9,668 3 View