Prasad Sulkshane

2 Questions 3 Answers 0 Followers

Questions related from Prasad Sulkshane

Can I use lentiviral packaging plasmids (pMD2.G and pPAX2) with a retroviral target plasmid?

Will the viral packaging work with a combination of a retroviral target plasmid and lentiviral packaging plasmids (pMD2.G and pPAX2)? Although I saw Syncytia formation in the HEK293FT cells post...

01 January 2019 4,928 3 View

What is the knockout efficiency of pSp-Cas9(BB)2A-Puro system?

I am using the pSp-Cas9(BB)2A-Puro system for creating a knockout cell line. My question is after the drug selection of the transfected cells, is it advisable to pool together all the colonies or...

10 October 2018 2,701 3 View