Prasad Dandekar

3 Questions 1 Answers 0 Followers

Questions related from Prasad Dandekar

Why to use maltopentaose in kinetics experiments (and Km,Ki calculations)involving alpha amylase? Why starch is not used?

For kinetics, can amylose or amylopectin be used? Why maltopentaose is used? Why not starch? Seen some papers which mention Km and Ki values using maltopentaose as substrate. Why?

21 November 2017 2,147 2 View

What should be sample sets for animal studies experiment?

I have isolated a pure compound from a medicinal plant and now want to check its activity in mice models. In this case, what samples should I to take into account from the following?: 1. crude...

07 September 2017 175 5 View

Purification of a doublet peak from reverse phase C18 smiprep/anal HPLC

Hi , I intend to separate a doublet of peak eluting from reverse phase HPLC column, along with the main peak, a shoulder peak appears which I want to get rid of the main peak. PDA detector at 245...

21 July 2017 4,112 4 View