How to get pure Plasmid DNA after alkaline lysis method? I am getting 260/280 reading around 1.1, how to sort it out?

I have isolated Plasmid DNA by alkaline lysis method (non-kit based). I also used Phenol:Chloroform:Isoamyl alcohol for removing the other contaminants but still getting the 260/280 reading around...

18 July 2019 2,438 0 View