Prakhar Agrawal

2 Questions 5 Answers 0 Followers

Questions related from Prakhar Agrawal

How do i remove non specific proteins binding to my protein of interest ?

my protein is fussed with GST tag (mol. wt ~ 52 kDa). i am using during Ni-NTA (binding in presence of 10 mM Imidazole and elution with 500 mM imidazole ). I am seeing non specific bands after...

11 April 2019 8,837 6 View

How to reconstitute heparin sodium salt powder ?

whether i should use normal autoclaved milliQ water or endotoxin free water or some buffer should be used for this work?

07 September 2017 6,344 1 View