Pradipta Das

2 Questions 2 Answers 0 Followers

Questions related from Pradipta Das

Should it really matter if we use Tris in place of HEPES as a component of lysis buffer for his-tag pull downs using Ni-NTA beads?

For making a general mammalian cell lysis buffer, I have been using HEPES as a component. However, I came across a paper where they have used Tris in place of HEPES while the rest of the...

29 April 2024 6,088 2 View

Struggling to get proper PLJM1 plasmid yield in E. coli DH5 alpha cells. Anyone got any suggestions?

I have been working with E. coli DH5 alpha competent cells to amplify my PLJM1 plasmid with an insert, but it just doesn't get amplified in the appropriate amount! The bacterial yield is too low...

14 January 2024 8,852 3 View