Pradip Kumar Singh

2 Questions 3 Answers 0 Followers

Questions related from Pradip Kumar Singh

How I can separate two protein/peptides having a slight difference?

I have a protein of 5.6 kda that was contaminated by its pro form (having 4 amino acids MKNM extra). Can I separate them using any type of chromatography? PI of both proteins are 6.7 and 6.9...

22 November 2012 9,411 5 View

How can I over express a protein in a wild type strain?

I want to over express a protein in the host strain (gram positive). What will be strategy for vector selection and transformation? Can I use an inducible promoter or not?

28 October 2012 8,031 4 View