What could be the possible reason for lack of Mtb protein expression cloned in pET28a even after induction ?

Need help!  I  cloned a 1440kb gene of Mycobacterium (Mtb)  in pET28a and transformed the BL21 cells. Gene sequence/ORF was checked.  Induced the culture at 0.6 O.D at 37°C with 0.1, 0.5 mM and...

13 December 2020 2,747 8 View