Prabuddha Sarkar

2 Questions 18 Answers 0 Followers

Questions related from Prabuddha Sarkar

Genomic DNA PCR amplification problem.

I have amplified one 3.1 Kb of fragment from genomic DNA, by using primers designed from the 5' and 3' UTR regions from the gene sequence to find out the introns in it. I have checked with the...

09 April 2012 2,568 38 View

Regarding the full length gene amplification.

I am facing big problems. I have got the full length gene sequence for my gene. Its 2500bp long approx. I have done 3 RACE s and got the full length by matching overlapping regions. Now I want to...

30 December 2011 7,085 14 View