Prabhu Thirupathi

3 Questions 5 Answers 0 Followers

Questions related from Prabhu Thirupathi

Why did my lyophilized proteins not work in ELISA, but gave a good result in WB?

I tried to concentrate my eluted proteins by lyophilization and then tried to use them in an ELISA experiment, but it did not work. The same protein still gives me a good band corresponding to the...

05 May 2014 2,497 8 View

Change in the cell morphology observed on flow cytometry after transfected with siRNA for knock down

I have a gene knocked down by siRNA technique in my stromal cells. After confirming the down regulation of the gene I have done a serial dilution of the cells to seed them on a 96 well plate for...

03 March 2013 9,597 5 View

Does anyone have frozen cells of anti-cMyc 9E10 hybridoma cell line?

I am working on recombinant proteins with cMyc tag expressed in HEK-Ebna cell lines. I need high quality antibody of anti-cMyc antibody to couple them against the CNBr beads and elute my...

12 December 2012 2,450 1 View