Prabhash Mahanta

2 Questions 5 Answers 0 Followers

Questions related from Prabhash Mahanta

Why did my transformed e.coli(BL21DE3) culture turn pink in LB broth media?

I cultured transformed BL21DE3 in LB broth from colonies. The colonies were pure white in color. The cells were transformed with my gene of interest. But the culture turned into pink. I have added...

06 March 2023 1,332 5 View

I am getting RNA bands only in plasmid isolation?

I am currently trying to isolate plasmid (pET- 32b) from transformed E.coli (BL21-DE3) cells. The cells have grown well on ampicillin agar plates. I am following Alkaline lysis method and Lithium...

19 October 2022 4,911 5 View