How does pre-running the SDS PAGE gel affect the discontinuous buffer system?

I am using 29:1 Acrylamide/Bis-acrylamide solution to prepare 12 % resolving gel and 4% stacking gel. The pH of the resolving and stacking gel buffers are pH 8.8 and pH 6.8 respectively. after...

16 February 2021 1,260 1 View