Poulami Sarkar

4 Questions 3 Answers 0 Followers

Questions related from Poulami Sarkar

How to do PCA analysis for NMR data?

I want to analyze few set of NMR data of two experimental sets. Can anyone help me with the PCA analysis, on how to do the anaysis and where?

11 May 2017 9,019 3 View

Is it necessary to remove the His tag from an expressed protein for production of antibody?

I have purified my protein (6kD) from pET28a+. I want to proceed with antibody production against this protein. Will the His Tag have any effect in the antibody production process?

21 July 2015 7,757 3 View

How an I identify 5-8KD his-tagged proteins from a cell lysate using SDS-PAGE?

I am unable to find any proper bands in the 15% gel lower to 10KD. My protein of interest is of 5-8KD. Is there any resolving problem in my gel?

29 December 2014 125 9 View

Can there be any difference if I use Rosetta instead of BL21?

Are there any advantages of the cell lines over each other, other than codon bias? Or any alternative can be used?

29 December 2014 1,618 8 View