Poovamma B C

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Questions related from Poovamma B C

How to express DPPH Antioxidant capacity of sample aa Ascorbic acid equivalent?

The Ascorbic acid standard of (0 to 200 micro gram/ml) was used for the assay. Should I plot graph of absorbance against concentration or %inhibition against concentration? Kindly help

14 March 2025 5,409 4 View

How to prepare blank for CUPRAC assay?

My reagent blank (1mL 10^‐2M CuCl2, 1mL 7.5 10^-3Mneocuproine and 1mL ammoniumacetate, the volume was made up with 1.1 ml distilled water)was darker than sample reaction mixtures. Can someone...

12 November 2024 5,101 2 View

Relationship between flavanoid expressed in quercetin equivalent and catechin equivalent?

How to correlate the values of TFC expressed in catechin equivalent to quercetin equivalent? Thank you in advance.

21 October 2024 5,528 1 View

How to prepare blank for CUPRAC assay?

My reagent blank (1mL 10^‐2M CuCl2, 1mL 7.5 10^-3Mneocuproine and 1mL ammoniumacetate, the volume was made up with 1.1 ml distilled water)was darker than sample reaction mixtures

20 September 2024 4,327 0 View

Frap reagent storability?

How long can we store FRAP reagent for antioxidant assay once it is prepared

08 September 2024 9,003 2 View

Frap assay protocol??

Kindly suggest how long should I incubate the sample mixture after adding FRAP reagent. The procedure I follow mentions to check absorbance after 4 min. But at 4 mins. The absorbance kept on...

05 September 2024 7,178 2 View