Does anyone has a detailed protocol for Lentiviral transduction of mESCs (grown on a feeder layer) including infection and then puromycin selection?

I am growing G4 mouse ESC on MEF (irradiated) layer and I wish to infect them with shRNA (lentiviral). Post infection I want to select the infected cells with puromycin. Subsequently, assess the...

25 February 2018 6,761 1 View