Pooranachithra Murugesan

2 Questions 6 Answers 0 Followers

Questions related from Pooranachithra Murugesan

If Ct value of a particular gene is undetermined during Real time PCR analysis in the sense, Would it be due to very less expression of that gene?

or would it be due to very high rated expression of that particular gene. Appreciate any valuable discussions regarding the above statement. Thanks in Advance...

04 April 2019 7,365 8 View

Can anyone suggest me a standard protocol for imaging Fura 2 AM staining and how to analyse the resulted pictures?

I am performing Fura 2 AM staining with a model nematode, C. elegans to monitor the calcium signaling and facing troubles in imaging them using CLSM. during imaging, Fura free and Fura saturated...

03 March 2019 9,158 2 View