Poonam Nagpal

3 Questions 4 Answers 0 Followers

Questions related from Poonam Nagpal

How to calculate siRNA concentration for Neon transfection ?

Hello, I am using Neon transfection system to transfect siRNA into suspension cell line. When calculating siRNA conc., does it have to based on tip volume or final volume. Although, i have an...

07 July 2018 8,885 3 View

For doing proliferation assay after SDC1 knockdown, how should I detach the cells from 24 well plate to do proliferation assay in 96 well plate?

I am planning to do proliferation assay in 96 well plate. I am concerned about the tranfected cells , will detaching the cells will affect the knockdown efficiency of SDC1 Any suggestions will be...

05 May 2017 9,821 3 View

Why do I detect proteins in starting material and flow through but not in elution lane in my Co-IP ?

I am getting strong bands for both bait and prey protein in starting material and flow through, only weak band in elution lane for bait protein and none for prey protein. I am using Pierce Co-IP...

07 July 2015 9,691 5 View