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Hi everyone, I am trying to transfect the GFP mRNA with dendrimers with 1:10 charge ratio. However I am unable to achieve the significant transfection as compared to lipofectamine. Please suggest...
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Hi We are working in the area of psoriatic diseases. We are everytime getting the problem in expression of protein in dermis. We unable to see the expression in epidermis. Even, we did IHC too...
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Dear sir/madam, Please suggest the biological assays which need to prove the drug and TNF Alfa binding studies. Thanks.
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Hi everybody, In our studies, we have used BALB/c mice for antipsoriatic activity evaluation. In that, we unable to get the proper tissue sections for histophathological and immunofluorescence...
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Hi everybody, I want to give the anti-miR as a therapeutic agent for the tumor bearing mouse. I have purchased the anti-miR from sigma Aldrich. Is there any standard protocol to deliver the...
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Hi everybody, I would like to transfect the anti-miR to the cancer cells. I have purchased it from sigma. I have the lipofectamine 2000 as a transfection agent.But I don't know the procedure to...
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We have normal in vitro facilities to culture the normal cancer cells. I want to work on cancer stem cells. Could you please suggest me what are the effective methods to maintain the cancer stem...
20 October 2015 8,581 4 View
I am going to detect miR-146a in human cell lines by RT PCR. I found most of the people are using the taqman assay kit. In my lab superscript III SYBR green method is already established and we...
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